Two New Fluorogenic Aptasensors Based on Capped Mesoporous Silica Nanoparticles to Detect Ochratoxin A
نویسندگان
چکیده
Aptamers have been used as recognition elements for several molecules due to their great affinity and selectivity. Additionally, mesoporous nanomaterials have demonstrated great potential in sensing applications. Based on these concepts, we report herein the use of two aptamer-capped mesoporous silica materials for the selective detection of ochratoxin A (OTA). A specific aptamer for OTA was used to block the pores of rhodamine B-loaded mesoporous silica nanoparticles. Two solids were prepared in which the aptamer capped the porous scaffolds by using a covalent or electrostatic approach. Whereas the prepared materials remained capped in water, dye delivery was selectively observed in the presence of OTA. The protocol showed excellent analytical performance in terms of sensitivity (limit of detection: 0.5-0.05 nm), reproducibility, and selectivity. Moreover, the aptasensors were tested for OTA detection in commercial foodstuff matrices, which demonstrated their potential applicability in real samples.
منابع مشابه
Oligonucleotide-capped mesoporous silica nanoparticles as DNA-responsive dye delivery systems for genomic DNA detection.
New hybrid oligonucleotide-capped mesoporous silica nanoparticles able to detect genomic DNA were designed.
متن کاملPluronic polymer capped biocompatible mesoporous silica nanocarriers.
A facile self-assembly method is described to prepare PEGylated silica nanocarriers using hydrophobic mesoporous silica nanoparticles and a pluronic F127 polymer. Pluronic capped nanocarriers revealed excellent dispersibility in biological media with cyto- and blood compatibilities.
متن کاملAu capped magnetic core/mesoporous silica shell nanoparticles for combined photothermo-/chemo-therapy and multimodal imaging.
Uniform Au NRs-capped magnetic core/mesoporous silica shell nanoellipsoids (Au NRs-MMSNEs) were prepared by coating a uniform layer of Au NRs on the outer surface of a magnetic core/mesoporous silica shell nanostructure, based on a two-step chemical self-assembly process. This multifunctional nanocomposite integrate simultaneous chemotherapy, photo-thermotherapy, in vivo MR-, infrared thermal a...
متن کاملTriggered release in lipid bilayer-capped mesoporous silica nanoparticles containing SPION using an alternating magnetic field.
We report here the on-command cargo controlled delivery using an alternating magnetic field (AMF) from magnetic silica mesoporous supports capped with a lipid bilayer.
متن کاملSynthesis of colloidal Janus nanoparticles by asymmetric capping of mesoporous silica with phenylsilsesquioxane.
Colloidal mesoporous silica nanoparticles asymmetrically capped with non-porous phenylsilsesquioxane have been prepared by adding phenyltriethoxysilane to an aqueous dispersion of mesostructured silica-surfactant composite nanoparticles. The integration of colloidal stability, mesoporosity and the Janus structure is quite promising for materials design applicable in various fields, including ca...
متن کامل